Ammonia (NH3)

Parameter Selection

Application Importance of Measuring Ammonia in Wine

Before fermentation, total nitrogen (present in the form of ammonia and amino acids) is commonly measured in must to ensure an adequate concentration of yeast available nitrogen (YAN) for efficient fermentation. Any ammonia not consumed by the yeast during fermentation remains in the finished wine. Ammonia in wine can impact flavor and microbial stability in the final product. Typical concentrations for ammonia in finished wine range between 3-50 mg/L.

Ammonia in wine is commonly measured with an ion-selective electrode (ISE). First, an ionic strength adjuster (ISA) is added to the wine. The ISA fixes the sample so that ion concentration, not just activity, can be measured. Additionally, the ammonia ISA contains a pH adjuster to buffer the sample above pH 10. Below pH 10, ammonia is present as the ammonium ion. Above pH 10, all ammonium in the wine is converted to ammonia gas (NH3). Ammonia ISEs use a gas permeable membrane, which selectively permits the passage of ammonia gas across. As ammonia diffuses across the membrane, it changes the pH of an internal electrolyte. A pH electrode housed inside the body of the ammonia ISE detects the pH change. The change in pH is directly proportional to the ammonia concentration.

ISE Meters

HI5000 Series Research Grade Laboratory Benchtops

The HI5000 series is our most advanced benchtop meters incorporating the latest microprocessor to bring exceptional features and performance for the most demanding user. These meters are completely customizable to user specific requirements. These research grade meters have many features including:


  • Ion Selective Electrode (ISE) input that displays measurement in various form of measurement units
  • Large color LCD that is sharp and easy to read
  • Capacitive Touch Keypad that is easy to clean
  • Logging and USB connection for data management
  • Two models to choose from:
    • 2 channel pH/ISE
    • 2 channel pH/ISE and Conductivity/TDS
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HI5000 Series Research Grade Laboratory Benchtops

The HI5000 series is our most advanced benchtop meters incorporating the latest microprocessor to bring exceptional features and performance for the most demanding user. These meters are completely customizable to user specific requirements. These research grade meters have many features including:


  • Ion Selective Electrode (ISE) input that displays measurement in various form of measurement units
  • Large color LCD that is sharp and easy to read
  • Capacitive Touch Keypad that is easy to clean
  • Logging and USB connection for data management
  • Two models to choose from:
    • 2 channel pH/ISE
    • 2 channel pH/ISE and Conductivity/TDS
Shop Now >

Portable pH/ISE meters

Portable meters provide the versatility to bring the meter to the sample. For users that are looking for an advanced version of a portable pH/ISE meter we offer the HI98191 with the features and performance of a benchtop.


Professional Portable Meter Features:

  • GLP data for tracking calibration data
  • CAL Check Diagnostics
  • USB for data transfer
  • Choice of ISE concentration units
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Electrodes

Ammonia ISE

HI4101 is a gas diffusion ion Selective Electrodes (ISE) for the measurement of ammonia in a wine sample. HI4101 uses a universal BNC connector compatible with meters that have BNC input.

  • Gas diffusion ISE
    • Samples treated with ISA to convert all ammonium to ammonia gas which then diffuses through a gas permeable membrane
  • Replaceable loose PTFE membranes
    • Less expensive that preformed membrane caps
  • BNC connector
    • Universal connector that can be used with any meter that accepts a BNC connection
Shop Now >

Ammonia ISE

HI4101 is a gas diffusion ion Selective Electrodes (ISE) for the measurement of ammonia in a wine sample. HI4101 uses a universal BNC connector compatible with meters that have BNC input.

  • Gas diffusion ISE
    • Samples treated with ISA to convert all ammonium to ammonia gas which then diffuses through a gas permeable membrane
  • Replaceable loose PTFE membranes
    • Less expensive that preformed membrane caps
  • BNC connector
    • Universal connector that can be used with any meter that accepts a BNC connection
Shop Now >

ISE Solutions & Accessories

Solutions for Ammonia ISE Measurement

Ammonia ISE measurement require the use of an Ionic Strength Adjuster (ISA), calibration standard and reference fill solution. All solutions are produced by Hanna and not sourced from third parties in order to control quality and cost.


  • One stop source for all solutions required to perform a Ammonia ISE measurement
  • Products produced to ISO 9001standards
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Solutions for Ammonia ISE Measurement

Ammonia ISE measurement require the use of an Ionic Strength Adjuster (ISA), calibration standard and reference fill solution. All solutions are produced by Hanna and not sourced from third parties in order to control quality and cost.


  • One stop source for all solutions required to perform a Ammonia ISE measurement
  • Products produced to ISO 9001standards
Shop Now >

Accessories for Ammonia ISE Measurement

Ammonia ISE accessories include replaceable PTFE membranes, pH buffers with chloride background for checking the inner pH electrode, and the individual components used in construction of the electrode.

  • Loose PTFE membranes that are lower cost than preformed membrane
  • Buffers with chloride background to check the functionality of the inner pH electrode
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Resources

Check out our Media Library and Knowledge Base pages specifically for wine and wine related products.